arabidopsis ath1 affymetrix gene chip (Thermo Fisher)
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Arabidopsis Ath1 Affymetrix Gene Chip, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gene+chip+ath1/pmc03046483-286-7-9
Average 86 stars, based on 1 article reviews
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1) Product Images from "Predictive network modeling of the high-resolution dynamic plant transcriptome in response to nitrate"
Article Title: Predictive network modeling of the high-resolution dynamic plant transcriptome in response to nitrate
Journal: Genome Biology
doi: 10.1186/gb-2010-11-12-r123
Figure Legend Snippet: High-resolution kinetics of transcriptome responses to NO 3 - treatment . (a) Levels of mRNA for nitrogen-responsive sentinel genes in Arabidopsis roots in response to NO 3 - treatment. Fourteen-day-old plants grown in the presence of ammonium succinate were treated with 1 mM KNO 3 or KCL (as a mock treatment). Plants were collected at 0 minutes (before treatment) and 3, 6, 9, 12, 15, 20, 25, 35, 45, and 60 minutes after treatment. Sentinel transcripts were measured in RNA from roots using RT-QPCR and normalized to two housekeeping genes (see Materials and methods). The insets show the Affymetrix MAS5 normalized signal for the sentinel genes on the 0- to 20-minute samples. The data represent the mean ± standard error of three and two biological replicates for QPCR and Affymetrix measurements, respectively. (b) Percentage of genes not detected as NO 3 - regulated in Wang et al . . (c) Overall behavior (relative expression) of 550 regulated genes (Log base 2(Signal KNO 3 /Signal KCl)) between 0 and 20 minutes. These data correspond to ATH1 measurement of the samples collected for the RT-PCR presented in (a) (grey shades; see also Materials and methods for further details).
Techniques Used: Quantitative RT-PCR, Expressing, Reverse Transcription Polymerase Chain Reaction
Figure Legend Snippet: Clustering analysis and QPCR reveals different patterns of expression in response to short-term NO 3 - treatment . (a) Cluster analysis of the relative expression of 550 regulated genes (Log base 2(Signal KNO 3 /Signal KCl)) between 0 and 20 minutes. These data correspond to ATH1 measurement of the samples collected for the RT-PCR shown in Figure 1 (see Materials and methods for further details). For clusters including genes with a significant over-representation of biological functions see Additional file . (b) Examples of three different gene behaviors (transitory, early, late responses) after NO 3 - provision.
Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction
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